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Rebeca Miranda‐Castro Noemí de‐los‐Santos‐Álvarez María Jesús Lobo‐Castañón Arturo J. Miranda‐Ordieres Paulino Tuñón‐Blanco 《Electroanalysis》2009,21(19):2077-2090
The use of nucleic acid with a specific sequence and a highly ordered secondary structure such as hairpins, quadruplexes and pseudoknots as biological recognition elements and switches in biosensors is rapidly increasing because of their improved features (e.g. selectivity) when compared with the traditional linear probes. Owing to the novelty, a critical outlook of their characteristics and a compilation of the latest advances are lacking. This article describes the potential of those nucleic acids probes whose molecular recognition ability relies on a conformational change (e.g. folding/unfolding mechanism) in electrochemical sensing. It provides an overview of the toolbox of assays using these probes for genosensors and aptasensors, highlighting its performance characteristics and the prospects and challenges for biosensor design. 相似文献
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Rebeca Marcilla Estibalitz Ochoteco Cristina Pozo‐Gonzalo Hans Grande Jose A. Pomposo David Mecerreyes 《Macromolecular rapid communications》2005,26(14):1122-1126
Summary: Polypyrrole (PPy), polyaniline (PANI), and poly(ethylenedioxythiophene) (PEDOT) aqueous dispersions were prepared by polymerizing the corresponding monomer in the presence of a polymeric ionic liquid (PIL), poly(1‐vinyl‐3‐ethylimidazolium bromide). By addition of bispentafluoroethanesulfonimide lithium salt, the PIL stabilizer becomes hydrophobic and precipitates in water and traps the conducting polymer microparticles inside. The dispersion of the recovered powders in organic solvents leads to organic conducting dispersions. After casting the organic dispersions, hydrophobic films with electrical conductivity values as high as 0.1 S · cm−1 were obtained.